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Comparative proteomic analysis of cold responsive proteins in two wheat cultivars with different tolerance to spring radiation frost

Shujuan ZHANG,Guoqi SONG,Yulian LI,Jie GAO,Jiao WANG,Guiju CHEN,Haosheng LI,Genying LI,Zhendong ZHAO

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 1,   Pages 37-45 doi: 10.15302/J-FASE-2014008

Abstract: degradation and signal transduction were isolated and identified by two-dimensional electrophoresis and MALDI-TOF-TOFMS.

Keywords： common wheat     spring radiation frost     proteomic analysis     2-DE     MALDI-TOF-TOF MS    

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Etiology of invasive candidosis agents in Russia: a multicenter epidemiological survey

N.V. Vasilyeva, E.R. Raush, M.V. Rudneva, T.S. Bogomolova, A.E. Taraskina, Yong Fang, Fengmin Zhang, N.N. Klimko

Frontiers of Medicine 2018, Volume 12, Issue 1,   Pages 84-91 doi: 10.1007/s11684-017-0612-x

Abstract: The species were identified by DNA sequencing and MALDI-TOF mass-spectrometry.

Keywords： prevalence     &Scy     andida species     invasive candidosis     nosocomial     etiology     MALDI-TOF mass-spectrometry    

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Determination of persistent organic pollutants by gas chromatography/laser multiphoton ionization/time-of-flight mass spectrometry

Osamu SHITAMICHI, Taiki MATSUI, Yamei HUI, Weiwei CHEN, Totaro IMASAKA

Frontiers of Environmental Science & Engineering 2012, Volume 6, Issue 1,   Pages 26-31 doi: 10.1007/s11783-011-0374-7

Abstract: An authentic mixture of polychlorinated biphenyls was measured using a short wide-bore capillary column for the group separation of major components present in an actual sample of Kanechlor. The limits of detection were improved by ca. 2 fold in comparison with those obtained using a conventional capillary column, since the retention time was reduced and the amount of analytes introduced into the mass spectrometer per unit time could be increased. On the other hand, surface-water and sediment samples containing polycyclic aromatic hydrocarbons (PAHs) were collected from the river located in the vicinity of a waste water treatment plant. Even acenaphthylene, a minor component of the mixture could be measured for the sediment sample, and the concentrations were determined for several heavy PAHs. As demonstrated, a technique involving laser multiphoton ionization/time-of-flight mass spectrometry was useful as a sensitive and selective analytical tool for the trace analysis of persistent organic pollutants in an environmental sample.

Keywords： polycyclic aromatic hydrocarbons (PAHs)     laser multiphoton ionization/time-of-flight mass spectrometry (MPI/TOF-MS    

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Transient imaging with a time-of-flight camera and its applications

Jing-yu Lin, Ri-hui Wu, Hong-man Wang, Ye-bin Liu,jylin@gxu.edu.cn,liuyebin@tsinghua.edu.cn

Frontiers of Information Technology & Electronic Engineering 2017, Volume 18, Issue 9,   Pages 1268-1276 doi: 10.1631/FITEE.1700556

Abstract: is a technique in photography that records the process of light propagation before it reaches a stationary state such that events at the light speed level can be observed. In this review we introduce three main models for with a : correlation model, frequency-domain model, and compressive sensing model. applications usually involve resolving the problem of light transport and separating the light rays arriving along different paths. We discuss two of the applications: imaging objects inside and recovering both the shape and texture of an object around a corner.

Keywords： Transient imaging     Time-of-flight (ToF) camera     Scattering media     Around corners    

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Transient imaging with a time-of-flight cameraand its applications

Jing-yu LIN, Ri-hui WU, Hong-man WANG, Ye-bin LIU

Frontiers of Information Technology & Electronic Engineering 2017, Volume 18, Issue 9,   Pages 1268-1276 doi: .org/10.1631/FITEE.1700556

Abstract: In thisreview we introduce three main models for transient imaging with atime-of-flight (ToF) camera:

Keywords： Transient imaging     Time-of-flight(ToF) camera     Scattering media     Around corners    

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Characterization of submicron aerosols in the urban outflow of the central Pearl River Delta region of China

Zhaoheng GONG, Zijuan LAN, Lian XUE, Liwu ZENG, Lingyan HE, Xiaofeng HUANG

Frontiers of Environmental Science & Engineering 2012, Volume 6, Issue 5,   Pages 725-733 doi: 10.1007/s11783-012-0441-8

Abstract: Delta in South China, using an Aerodyne High-Resolution Time-of-Flight Aerosol Mass Spectrometer (HR-ToF-AMS

Keywords： organic aerosol     high-resolution time-of-flight aerosol mass spectrometer (HR-ToF-AMS)     positive matrix    

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Analysis of Brucella cellular fatty acids

Zhen-Xiang ZHAO MS, Hai JIANG MD, Bu-Yun CUI MS, Hong-Yan ZHAO, Dong-Ri PIAO MS, Lan-Yu LI, Su-Zhen HAOMS,

Frontiers of Medicine 2010, Volume 4, Issue 2,   Pages 216-219 doi: 10.1007/s11684-010-0037-2

Abstract: To acquire data of Brucella cellular fatty acids (CFAs) and probe into the possibility of utilizing CFAs information in typing Brucella, 19 reference strains were subjected to CFAs study. After all strains were inoculated on Brucella Agar plates, the cells were harvested, saponificated, methylated and extracted to provide fatty acid methylesters for gas chromatography (GC) analysis. Based on the CFAs data matrix, a dendrogram of 19 reference strains was generated by SPSS11.5 software package. The results showed that 19 reference strains were divided into five clusters: cluster 1 included (bv. 1, 2, 3, 5) and ; cluster 2 included (bv. 3, 4, 5, 6) and (bv. 1, 2, 3); cluster 3 included (bv. 1, 2, 7, 9) and ; cluster 4 was (bv. 4); and cluster 5 was . Typing Brucella by GC analysis of CFAs is a good method to reflect drug resistance of Brucella, and the classification is beneficial for clinical therapy. It also provides a new result of typing and demonstrates that the traditional classification is not completely reasonable. CFAs analysis may identify (bv. 4) and .

Keywords： Brucella     gas chromatography     cellular fatty acids     dendrogram     typing    

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Preparation, identification, and clinical application of anti-HBs monoclonal antibody that binds both wild-type and immune escape mutant HBsAgs

Fanghe LI BM , Chunyan ZHANG MS , Jinghua LIU MS , Xiaoyan ZHANG MS , Bing YAN , Bo ZHANG MS , YongguoHUANG MS , Jingsong GONG BM , Yan CHEN BM ,

Frontiers of Medicine 2009, Volume 3, Issue 3,   Pages 277-283 doi: 10.1007/s11684-009-0047-0

Abstract: Using a standard cellular fusion technique and indirect enzyme-linked immunosorbent assay (ELISA), a hybridoma cell line strain secreting anti-HBs monoclonal antibody (mAb) (defined G6 mAb) was obtained. The cells grew and secreted mAb stably. Antibody titers in the culture supernatant and ascites were 2.048×10 and 4.096×10, respectively. By applying the anti-HBs G6 mAb and horseradish peroxidase (HRP)-labeled goat anti-HBs antibody, we developed a sandwich ELISA (defined G6m ELISA) for detecting both wild-type and immune escape mutant HBsAgs (IEM HBsAg). The assay was performed to detect 17 species of genome recombinant expression HBsAg, including two wild-type species and 15 IEM HBsAg species, which varied in the “a” determinant, in a group of patients infected with hepatitis B virus (HBV). The patients previously had a lower ELISA detection signal [(absorbance of patients/absorbance of normal people (P/N): 1.0―4.5)]. The results demonstrated that the sensitivity of this assay to wild-type HBsAg was no less than 0.125g/L; 12 of 15 IEM HBsAg species (P/N≥2.5) were positive for G6 mAb. Of the positive IEM HBsAg species, two had a low absorbance value at 450nm (), one had an intermediate value and nine had a high value, which was 7.55%(mean), 59.4% and 92.1%―109.4% of the wild-type value, respectively. The two species with low value and the three negative species mutated at the bases 120―124 in the first loop of the HBV “a” determinant. Using the G6 ELISA and two commercial ELISA kits (A and B), 177 patients were tested. The G6 ELISA had a significantly higher detection rate than either commercial ELISAs (19.21% 14.89% and 6.21%, respectively; <0.01, <0.05, respectively).

Keywords： hepatitis B virus     gene variation     hepatitis B surface antigen     immune escape     enzyme-linked immunosorbent assay    

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Study on factors affecting TB/HIV co-infection in four counties of China

Shi-Ming CHENG MD, Yu-Ji LAI MS, Er-Yong LIU MS, Lin ZHOU MD, Xue-Jing WANG MS, Qiu-Lan CHEN MS, Dong-MingLI MS, Ning WANG MD,

Frontiers of Medicine 2010, Volume 4, Issue 2,   Pages 185-191 doi: 10.1007/s11684-010-0040-7

Abstract: The factors affecting case detection of tuberculosis (TB)/human immunodeficiency virus (HIV) co-infection cases were explored in order to provide evidence-based formulation of a TB/HIV co-infection control strategy in China. Four counties with different modes of HIV transmission and varying cooperation mechanisms between TB and HIV institutions were selected. HIV test among TB patients and TB examination among people living with HIV/acquired immunodeficiency syndrome (AIDS) were conducted. Patients also completed questionnaires on potential factors affecting TB/HIV co-infection. The results showed that 19 (1.7%) were HIV-positive among 1089 cases of people living with active TB who received HIV testing. Conversely, there were 126 (10%) cases of active TB among 1255 cases of people living with HIV/AIDS. Among the newly discovered 145 cases of TB/HIV co-infection, AIDS institutions found 86.9% of these co-infections and 14.1% came from TB institutes. In different registration categories, there were 31 (21.4%) cases of smear positive TB, 104 (71.7%) cases of smear negative TB, and extrapulmonary cases accounted for 10 (6.9%). Gender, occupation, marital status, ethnic group, education, HIV transmission route, CD4 level, and type of TB susceptible symptoms had significant effects on whether HIV/AIDS patients were co-infected with TB disease (<0.01). Sex, age, ethnic group, occupation, and type of tuberculosis had no significant influence on whether TB patients had HIV infection. AIDS institutions could find more TB/HIV co-infection patients in comparison with TB institutions, particularly in patients with smear negative pulmonary tuberculosis and extrapulmonary tuberculosis. Therefore, a cooperation mechanism should be built up between TB and HIV control systems, and routine TB checks should be conducted for HIV/AIDS patients. In areas where sex or drug use is the major transmission mode of HIV infection, antiretroviral therapy should be expanded, treatment adherence should be improved and patients’ immunity level should be increased in order to decrease TB incidence. Cough and expectoration of more than two weeks, bloody sputum, and recurrent fevers could be revealing symptoms in TB screening of HIV/AIDS patients.

Keywords： tuberculosis/acquired immunodeficiency syndrome     dual infection     prevention and control     case detection     impact factors    

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LC/MS/MS and radioisotope method combined for recognizing the affinity between catalpol and OCT2 transporter

Zhiyu ZHANG, Changxiao LIU, Duanyun SI, Rong LU, Xiulin YI

Frontiers of Chemical Science and Engineering 2012, Volume 6, Issue 4,   Pages 436-442 doi: 10.1007/s11705-012-1222-4

Abstract: In this paper, chromatography/tandem mass spectrometry (LC/MS/MS) combined with radioisotope method wasAnd samples were precipitated with methanol and quantified with LC/MS/MS.

Keywords： catalpol     LC/MS/MS     OCT2    

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A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host, Pomacea canaliculata

Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,

Frontiers of Medicine 2010, Volume 4, Issue 2,   Pages 204-207 doi: 10.1007/s11684-010-0043-4

Abstract: Pooling field specimens could reduce the number of assay and thus increase the efficiency in detecting and screening pathogen infections by polymerase chain reaction (PCR)-based assay. We investigated a pooling strategy in diagnosis of in Two settings of specimens were prepared, divided into portions and detected by multiplex PCR. Specimens A was 0.4490 g positive lung tissue of 28 larval nodes from four snails mixed with 1.310€g negative lung tissue from six snails and divided into 32 portions. Specimens B was 0.5448 g positive lung tissue with 26 larval nodes from two snails mixed with 1.092 g negative lung tissue from seven snails and divided into 48 portions. Repeated sampling was performed and sample size-accumulated positive rate curves were drawn. According to the sample size-accumulated positive rate curves, the appropriate sample size of the two specimens were 18 and 15, respectively, which is 0.36–0.58 to the total sample size. These test characteristics and the relevant factors to the sample size would need to be determined in much larger studies and more appropriately in field populations. The result indicates that the number of larval node is not the most important, nor the only factor to the sample size. It also implies the feasibility to detect in by pooling strategies.

Keywords： Angiostrongylus cantonensis     Pomacea canaliculata     multiplex polymerase chain reaction     diagnosis     pooling    

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The Physical Precursors for the Ms 8.7 and Ms 8.5 Earthquakes in Indian Ocean

Sun Wei,Sun Xiaoming

Strategic Study of CAE 2008, Volume 10, Issue 2,   Pages 14-25

Abstract: precursors-the gestation process and the signal of impending earthquake of the Indian Ocean Earthquake of Msthat there are great differences for the stress in crust between before and after the eart hquake of Ms8-7 and that of Ms 8-5.tsunami is analyzed.The sign of underground material migration is also found in the gestation process of Ms

Keywords： Ms 8-7 and Ms 8-5 earthquake in Indian Ocean     earthquqke precursor     signal of impending earthquake     tsunami    

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The inactivation of bacteriophages MS2 and PhiX174 by nanoscale zero-valent iron: Resistance difference

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 8, doi: 10.1007/s11783-022-1529-4

Abstract:

• The resistance of phage PhiX174 to nZVI was much stronger than that of MS2

Keywords： Nanoscale zero-valent iron (nZVI)     MS2     PhiΧ174     Resistance     Inactivation     Pathogenic microorganisms    

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Mature cystic teratoma of the parotid gland: a case report and review of the literature

Lenan SHAO DMD, MD, Hanxiong GUAN MD, Jie WAN MS,

Frontiers of Medicine 2009, Volume 3, Issue 4,   Pages 503-506 doi: 10.1007/s11684-009-0076-8

Abstract: We report one case of mature cystic teratoma that presented as a tender, well-defined round mass in the right parotid gland region. Teratomas are common neoplasms but are very rarely found in the parotid gland region, so diagnosis and treatment when such occurs is a challenge. Magnetic resonance imaging scanning is useful in determining the nature of teratomas, and only surgery and pathology can provide a final diagnosis. The treatment strategy is to preserve both the facial nerve and the surrounding parotid gland tissue, while completely extirpating the teratoma.

Keywords： teratoma     parotid gland    

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Perfluoroalkane acids in human milk under the global monitoring plan of the Stockholm Convention on Persistent Organic Pollutants (2008–2019)

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 10, doi: 10.1007/s11783-022-1541-8

Abstract:

● Perfluorooctanesulfonic acid and perfluorooctanoic acid highest in human milk.

Keywords： Human biomonitoring     Human breast milk     LC-MS/MS analysis     Lifestyle parameters    

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Title Author Date Type Operation

Comparative proteomic analysis of cold responsive proteins in two wheat cultivars with different tolerance to spring radiation frost

Shujuan ZHANG,Guoqi SONG,Yulian LI,Jie GAO,Jiao WANG,Guiju CHEN,Haosheng LI,Genying LI,Zhendong ZHAO

Journal Article

Etiology of invasive candidosis agents in Russia: a multicenter epidemiological survey

N.V. Vasilyeva, E.R. Raush, M.V. Rudneva, T.S. Bogomolova, A.E. Taraskina, Yong Fang, Fengmin Zhang, N.N. Klimko

Journal Article

Determination of persistent organic pollutants by gas chromatography/laser multiphoton ionization/time-of-flight mass spectrometry

Osamu SHITAMICHI, Taiki MATSUI, Yamei HUI, Weiwei CHEN, Totaro IMASAKA

Journal Article

Transient imaging with a time-of-flight camera and its applications

Jing-yu Lin, Ri-hui Wu, Hong-man Wang, Ye-bin Liu,jylin@gxu.edu.cn,liuyebin@tsinghua.edu.cn

Journal Article

Transient imaging with a time-of-flight cameraand its applications

Jing-yu LIN, Ri-hui WU, Hong-man WANG, Ye-bin LIU

Journal Article

Characterization of submicron aerosols in the urban outflow of the central Pearl River Delta region of China

Zhaoheng GONG, Zijuan LAN, Lian XUE, Liwu ZENG, Lingyan HE, Xiaofeng HUANG

Journal Article

Analysis of Brucella cellular fatty acids

Zhen-Xiang ZHAO MS, Hai JIANG MD, Bu-Yun CUI MS, Hong-Yan ZHAO, Dong-Ri PIAO MS, Lan-Yu LI, Su-Zhen HAOMS,

Journal Article

Preparation, identification, and clinical application of anti-HBs monoclonal antibody that binds both wild-type and immune escape mutant HBsAgs

Fanghe LI BM , Chunyan ZHANG MS , Jinghua LIU MS , Xiaoyan ZHANG MS , Bing YAN , Bo ZHANG MS , YongguoHUANG MS , Jingsong GONG BM , Yan CHEN BM ,

Journal Article

Study on factors affecting TB/HIV co-infection in four counties of China

Shi-Ming CHENG MD, Yu-Ji LAI MS, Er-Yong LIU MS, Lin ZHOU MD, Xue-Jing WANG MS, Qiu-Lan CHEN MS, Dong-MingLI MS, Ning WANG MD,

Journal Article

LC/MS/MS and radioisotope method combined for recognizing the affinity between catalpol and OCT2 transporter

Zhiyu ZHANG, Changxiao LIU, Duanyun SI, Rong LU, Xiulin YI

Journal Article

A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host, Pomacea canaliculata

Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,

Journal Article

The Physical Precursors for the Ms 8.7 and Ms 8.5 Earthquakes in Indian Ocean

Sun Wei,Sun Xiaoming

Journal Article

The inactivation of bacteriophages MS2 and PhiX174 by nanoscale zero-valent iron: Resistance difference

Journal Article

Mature cystic teratoma of the parotid gland: a case report and review of the literature

Lenan SHAO DMD, MD, Hanxiong GUAN MD, Jie WAN MS,

Journal Article

Perfluoroalkane acids in human milk under the global monitoring plan of the Stockholm Convention on Persistent Organic Pollutants (2008–2019)

Journal Article